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The question of at what resolution the large eddy simulations (LESs) of a tropical cyclone (TC) high wind area may converge largely remains unanswered. To address this issue, LESs with five resolutions of 300 m, 100 m, 60 m, 33 m, and 20 m are performed in this study to simulate a high wind area near the radius of maximum wind of Typhoon Chanthu (2021) using the Weather Research and Forecasting (WRF) model. The results show that, for a limited area LES, model grid resolution may alter the local turbulence structure to generate significantly different extreme values of temperature, moisture, and winds, but it only has a marginal impact on the median values of these variables throughout the vertical column. All simulations are able to capture the turbulent roll vortices in the TC boundary layer, but the structure and intensity of the rolls vary substantially in different resolution simulations. Local hectometer-scale eddies with vertical velocities exceeding 10 m s−1 are only observed in the 20 m resolution simulation but not in the coarser resolution simulations. The ratio of the resolved turbulent momentum fluxes and turbulent kinetic energies (TKEs) to the total momentum fluxes and TKEs appears to show some convergence of LESs when the grid resolution reaches 100 m or finer, suggesting that it is an acceptable grid resolution for LES applications in TC simulations. 

Abstract Polyethylene glycol (PEG) is a polymer routinely used to modify biologics and nanoparticles to prolong blood circulation and reduce immunogenicity of the underlying therapeutic. However, several PEGylated therapeutics induce the development of anti-PEG antibodies (APA), leading to reduced efficacy and increased adverse events. Given the highly flexible structure of PEG, how APA specifically bind PEG remains poorly understood. Here, we report a crystal structure illustrating the structural properties and conformation of the APA 6-3 Fab bound to the backbone of PEG. The structure reveals an open ring-like sub-structure in the Fab paratope, whereby PEG backbone is captured and then stabilized via Van der Waals interactions along the interior and exterior of the ring paratope surface. Our finding illustrates a strategy by which antibodies can bind highly flexible repeated structures that lack fixed conformations, such as polymers. This also substantially advances our understanding of the humoral immune response generated against PEG.